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polymer based mirus transit x2 transfection  (Mirus Bio)


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    Structured Review

    Mirus Bio polymer based mirus transit x2 transfection
    PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with <t>Mirus</t> <t>TransIT-X2</t> (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .
    Polymer Based Mirus Transit X2 Transfection, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 98/100, based on 2313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirus+transit+x2/TransIT-X2/pmc13076212-152-28-29
    Average 98 stars, based on 2313 article reviews
    polymer based mirus transit x2 transfection - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists"

    Article Title: Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag328

    PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .
    Figure Legend Snippet: PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .

    Techniques Used: Activation Assay, In Vitro, Transfection, Expressing, High Molecular Weight, Standard Deviation

    Related Articles

    Transfection:

    Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1.
    Article Snippet: .. Twenty-four hours after plating, the cells were transfected with the following combination of vectors: pxR003 gRNA vector (200 ng) + pxR001 EF1a-CasRx-2AEGFP (active CasRx, 200 ng) (109049, Addgene) using Mirus TransIT-X2 ® (MIR6004, Mirus Bio). ..

    Article Title: Compositions and methods for nucleic acid modifications
    Article Snippet: .. Editing Activity in Human Cells Nucleases were tested for activity in HEK293T cells following plasmid transfection using Mirus Transit X2 reagent. .. Using the vectors constructed in Example 1, tests were performed in 96 well plates transfected with 150 ng of nuclease expression vector and 50 ng of targeting guide vector following the Mirus Transit X2 transfection recommendations.

    Article Title: Nesprin-2 contains BH3-like motifs that can promote cell death
    Article Snippet: .. Four hours after plasmid transfection using the Mirus TransIT-x2 reagent, media in each well was replaced with media containing 10 μM quinoline-VaL-Asp(OMe)-CH2-OPH (Q-VD-OPH, Apex Biotechnology) to remove the Mirus reagent and inhibit caspase activation. ..

    Article Title: Integration of Nuclear Receptors into a Schwann cell Gene Regulatory Network
    Article Snippet: S16 Schwann cells and Primary Rat Schwann cells (RSCs) were cultured in 6-well plates in Corning DMEM containing 4.5 g/L glucose and L-glutamine without sodium pyruvate (#10-017-CV) and 5% bovine growth serum (Hyclone). .. S16s were transfected with control siRNA (IDT DS NC1), or siRNAs for Nr2f1 (IDT, RNC.RNAI.N031130.12.3), Nr2f2 (IDT RNC.RNAI.N080778.12.1), using Mirus Transit X2 (MIR 6000), as described ( ). .. A repeat of the Nr2f experiment was done with independent Nr2f siRNA’s (n=3) from IDT using the AMAXA 4D nucleofector with the AMAXA SE Cell Line 4D Nucleofector Kit L (Lonza).

    Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1
    Article Snippet: .. Twenty-four hours after plating, the cells were transfected with the following combination of vectors: pxR003 gRNA vector (200 ng) + pxR001 EF1a-CasRx-2A-EGFP (active CasRx, 200 ng) (109049, Addgene) using Mirus TransIT-X2 ® (MIR6004, Mirus Bio). ..

    Article Title: Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter
    Article Snippet: SHSY5Y cells were seeded at 6 × 10 one day before transfection and transfection was performed using Mirus TransIT-X2 (Fisher Scientific, Cat #MIR 6000). .. Transfection was then performed as previously described using Mirus TransIT-X2. .. SHSY5Y cells were grown to confluence in 35mm glass bottom 6-well plates (Fisher Scientific, Cat #NC0452316) in EMEM containing 10 μM retinoic acid (RA, Sigma-Aldrich, Cat #R2625) for 3 days followed by removal of the RA-containing media and replaced with EMEM containing 80 nM 12-o-tetradecanoyl-phorbol-13-acetate (TPA, Millipore Sigma, Cat #5.00582.0001) for another 3 days of differentiation.

    Activity Assay:

    Article Title: Compositions and methods for nucleic acid modifications
    Article Snippet: .. Editing Activity in Human Cells Nucleases were tested for activity in HEK293T cells following plasmid transfection using Mirus Transit X2 reagent. .. Using the vectors constructed in Example 1, tests were performed in 96 well plates transfected with 150 ng of nuclease expression vector and 50 ng of targeting guide vector following the Mirus Transit X2 transfection recommendations.

    Plasmid Preparation:

    Article Title: Compositions and methods for nucleic acid modifications
    Article Snippet: .. Editing Activity in Human Cells Nucleases were tested for activity in HEK293T cells following plasmid transfection using Mirus Transit X2 reagent. .. Using the vectors constructed in Example 1, tests were performed in 96 well plates transfected with 150 ng of nuclease expression vector and 50 ng of targeting guide vector following the Mirus Transit X2 transfection recommendations.

    Article Title: Nesprin-2 contains BH3-like motifs that can promote cell death
    Article Snippet: .. Four hours after plasmid transfection using the Mirus TransIT-x2 reagent, media in each well was replaced with media containing 10 μM quinoline-VaL-Asp(OMe)-CH2-OPH (Q-VD-OPH, Apex Biotechnology) to remove the Mirus reagent and inhibit caspase activation. ..

    Activation Assay:

    Article Title: Nesprin-2 contains BH3-like motifs that can promote cell death
    Article Snippet: .. Four hours after plasmid transfection using the Mirus TransIT-x2 reagent, media in each well was replaced with media containing 10 μM quinoline-VaL-Asp(OMe)-CH2-OPH (Q-VD-OPH, Apex Biotechnology) to remove the Mirus reagent and inhibit caspase activation. ..

    Control:

    Article Title: Integration of Nuclear Receptors into a Schwann cell Gene Regulatory Network
    Article Snippet: S16 Schwann cells and Primary Rat Schwann cells (RSCs) were cultured in 6-well plates in Corning DMEM containing 4.5 g/L glucose and L-glutamine without sodium pyruvate (#10-017-CV) and 5% bovine growth serum (Hyclone). .. S16s were transfected with control siRNA (IDT DS NC1), or siRNAs for Nr2f1 (IDT, RNC.RNAI.N031130.12.3), Nr2f2 (IDT RNC.RNAI.N080778.12.1), using Mirus Transit X2 (MIR 6000), as described ( ). .. A repeat of the Nr2f experiment was done with independent Nr2f siRNA’s (n=3) from IDT using the AMAXA 4D nucleofector with the AMAXA SE Cell Line 4D Nucleofector Kit L (Lonza).

    Concentration Assay:

    Article Title: Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists
    Article Snippet: .. Complexation with Mirus TransIT-X2: 3 μl TransIT-X2 reagent was added to 97 μl solution containing polyRNAs or hybrids dissolved in Opti-MEM, giving a final concentration of 5 or 10 ng/μl (calculated based on the polyRNA concentration in the mixture). ..

    other:

    Article Title: Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter
    Article Snippet: U2OS and HeLa cells were seeded at 3 × 10 one day before transfection in 35mm glass bottom dishes (Cellvis, Cat #d35-14-1.5-n).



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    Image Search Results


    PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .

    Journal: Nucleic Acids Research

    Article Title: Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists

    doi: 10.1093/nar/gkag328

    Figure Lengend Snippet: PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .

    Article Snippet: As we observed that activation levels by polyRNAs in different reporter cell lines are influenced by the transfection reagent, we used either the commercial lipid-based Lipofectamine 3000 or polymer-based Mirus TransIT-X2 transfection reagent, as optimized for each cell line.

    Techniques: Activation Assay, In Vitro, Transfection, Expressing, High Molecular Weight, Standard Deviation

    PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .

    Journal: Nucleic Acids Research

    Article Title: Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists

    doi: 10.1093/nar/gkag328

    Figure Lengend Snippet: PolyRNAs of varying structures and sequences activate multiple PRRs. ( A ) Proposed structural features of polyRNA for PRR-specific recognition and activation. A dumbbell-shaped DNA template is processively transcribed by T7 RNA polymerase to generate 5′ triphosphate-containing polyRNA structures comprising repeat units of dsRNA and ssRNA regions. Panel of polyRNAs screened for activation of specific PRRs. All polyRNAs in the panel have a 25 bp dsRNA stem in each repeat, with varying ssRNA loop and connecting region lengths and sequences as indicated in the schematics. GUU labels indicate GU-rich ssRNA sequences. ( C, D ) Co-transcriptional structure prediction of monomeric units ( C ) and oligomeric RNAs ( D ) by KineFold . Pseudoknots are visualized as coloured single-stranded regions connected by two straight lines as predicted by KineFold. Structure prediction images were created with KineFold and polished using Adobe Photoshop. ( E ) In vitro activation of PRRs by polyRNAs transfected with Mirus TransIT-X2 (Mirus) in HEK-Blue hTLR3, hTLR7, and Null1 cells at 2 μg/ml. HEK-Blue Null1 is the parental cell line of HEK-Blue TLR cell lines, with baseline PRR expression levels. ( F ) In vitro RIG-I activation by polyRNAs transfected by Lipofectamine 3000 (Lipo) in HEK-Lucia RIG-I cells at 0.5 μg/ml. ( G ) In vitro IRF activation of IRF in RAW-Dual cells by polyRNAs transfected by Lipofectamine at 0.5 μg/ml. For panels (E)–(G), established agonist benchmarks were included for each PRR reporter cell line: high molecular weight poly(I:C) for TLR3, Null1, and RAW-Dual, R848 for TLR7, and 3p-hpRNA for RIG-I. The data represent the mean ± standard deviation of n = 3 technical replicates. Data were analysed by one-way Analysis of Variance (ANOVA) with Šidak’s multiple comparisons test. Ns, no significant difference between bracketed groups. **/***/**** denotes significance between bracketed groups ( P <.01/.001/.0001). Figure and were created in BioRender. Yang, Y. (2026) https://BioRender.com/gd4yhbl .

    Article Snippet: Complexation with Mirus TransIT-X2: 3 μl TransIT-X2 reagent was added to 97 μl solution containing polyRNAs or hybrids dissolved in Opti-MEM, giving a final concentration of 5 or 10 ng/μl (calculated based on the polyRNA concentration in the mixture).

    Techniques: Activation Assay, In Vitro, Transfection, Expressing, High Molecular Weight, Standard Deviation